Beckman Coulter Life Sciences Extracellular Vesicles Workshop

About the Event

Join us for an exciting workshop where you will have the opportunity to delve into both theoretical and practical aspects of our latest innovations.

Workshop Agenda:

12:00-13:00 — Theoretical Part
Location: Messe Wien Exhibition Congress Center, Messepl. 1, 1020 Wien, Austria

• Optimizing EV Sample Purification: Planning Density Gradient Ultracentrifugation (DG-UC) for Efficient Contaminant Removal
• Next-Generation EV Analysis: Flow Cytometry with CytoFLEX nano and Key Considerations 

15:30-16:10 bus transfer from Wien Messe to Vienna One Facility

16:30-18:30 — Practical Part
Location: Danaher Vienna One Facility, Hernalser Hauptstraße, 1170 Wien, Austria

• Hands-On with CytoFLEX nano: From Calibration to Real-World EV Sample Analysis

18:30 optional bus transfer from Vienna One Facility to Wien Messey

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Extracellular vesicles (EVs) are gaining increasing attention across various research fields, yet their analysis presents unique challenges due to their small size, heterogeneity, and complex isolation workflows. This workshop aims to equip researchers with essential knowledge and practical experience in optimizing EV workflows for flow cytometry-based analysis, with a special focus on the CytoFLEX nano flow cytometer.

In the afternoon, theoretical sessions will begin with a discussion on Density Gradient Centrifugation – a powerful approach to EV isolation. We will cover key considerations for planning EV sample preparation, with a focus on removing contaminants such as proteins, cell-free DNA, and lipoproteins. Additionally, you will receive updates on new tools designed to support the standardization of Density Gradient Ultracentrifugation.

Following this, we will introduce the audience to flow cytometry in EV research, focusing on the transition from conventional cytometry to next-generation instruments like the CytoFLEX nano. This session will cover key considerations for EV analysis, including detection limits, scatter resolution, and fluorescence sensitivity. Furthermore, participants will gain insights into the MISEV and MIFlowCyt-EV guidelines, which set reporting standards for EV research. We will demonstrate how the CytoFLEX nano aligns with these frameworks, enabling robust EV characterization with improved standardization and data comparability.

During the hands-on session in the evening, we will demonstrate the CytoFLEX nano live, working in three participant groups. The session will start with an overview of the instrument’s design, key components, and software functionality. The practical portion will showcase the system’s capabilities through two experiments. First, participants will measure NIST-traceable beads of different sizes and use this data to calibrate the instrument with FCMPASS software. After successful calibration, GFP-positive EVs from cell culture will be analyzed, and their size will be determined based on the calibrated data.

This hands-on course provides a comprehensive learning experience, allowing participants to explore how the CytoFLEX nano enhances EV research through high-resolution detection (40 nm polystyrene), automated cleaning routines, and internal background determination. The workshop is designed to give researchers practical insights into generating high-quality, reproducible EV data aligned with MIFlowCyt-EV guidelines.

Seats are limited, and priority will be given to registrants attending both the theory and practical sessions. Registration is only confirmed upon receipt of an email from our team. Selected participants will receive a confirmation email with logistical details, while those not selected will also be notified. Please wait for an email from Beckman before making any arrangements.