自動化細胞培養系統
Cydem VT 自動化細胞培養系統將多項 Beckman Coulter 技術整合到單一獨立平台中，為辛勤工作的實驗室管理者和科學家打開了全新的世界，他們每天都在細胞株開發的高壓世界中努力爭取成功。透過單一實驗從更多clone中獲得更多了解。
整合式生物反應器模組
- 24 層深孔生物反應器平板中平行培養 96 個clone
- 獨特的排氣芯片可實現個別排氣控制
- 在培養過程中保持最佳 pH 值和溶解氧水平
整合式細胞計數與存活率模組
- 在整個實驗過程中定期更新細胞計數和存活率
- 以較低的樣本量進行精確的測量
- 使用我們著名的 Vi-CELL BLU Analyzer 分析儀技術
整合滴定模組
- 定期測量抗體濃度
- 了解您clone 的生產力
- 對您的clone 選擇做出明智的決定
- 小樣品容量
整合式液體處理器
- 自動化您的克隆篩選
- 運行更受控制的fed-batch條件，降低選擇次佳clone的風險
- 自動seeding細胞密度規範化
- 為分析、fed-batch和pH值控制排成
高通量
96 x 5 mL 並行微型生物反應器
最佳化選擇信賴度
在每個平行微生 物反應器中，pH 值受控、批量供料條件下具有單獨氣相調節（CO2 和溶解氧）
早期洞察
在整個培養過程中定期測量甲板上的細胞數量、存活率和滴度
全自動化
減少手工操作和人為錯誤；
降低污染風險
3 天隨插即用自動化操作，可自動標準化細胞密度
- 完全整合的解決方案，具備現場細胞計數、細胞存活率、mAbs 滴度、pH 值和溶解氧測量
- 3 天自動化操作
- 自動接種細胞培養
- 分析測量、餵料策略及 pH 控制的排程建立工具
- 集中管理數據於單一儀器，並可輕鬆匯出為 .csv 格式
14 天內對 96 個clones進行篩選。顯示實驗過程中的存活細胞密度和 mAbs 濃度。為了更好地概觀，我們選擇了 10 個具有不同生長和生產性能的clone。
Reagents and Consumables
Human IgG Standard Kit, 10 mg
The Human IgG Standard Kit contains 10 mg human IgG standard for measuring a monoclonal antibody (mAb) standard curve with the Cydem VT system.
Cydem VT System Cell Health Test Kit
The Cell Health Test Kit contains reagents for 6 x 96 cell health tests (including Trypan Blue Dye, Cleaning Solution, and Cell Health Diluent). It is recommended if more cell count and viability measurements are required than are included in the regular Cell Culture Standard Experiment Kit.
Product not for use in diagnostic or therapeutic procedures.
Cydem VT System IgG Titer Standard Experiment Kit
The IgG Titer Standard Experiment Kit contains all reagents and titer plates required for a 2-week standard campaign: contains one Human IgG Standard Kit, 10 mg, and two Titer Test Kits (including Titer Diluent, Cleaning Agent, and Titer Plates) for a total of 10 x 96 antibody titer tests.
Product is not intended or validated for use in the diagnosis of disease or other conditions.
Cydem VT System IgG Titer Extended Experiment Kit
The IgG Titer Extended Experiment Kit contains all reagents and titer plates required for an extended campaign: Contains one Human IgG Standard Kit, 10 mg, and three Titer Test Kits (including Titer Diluent, Cleaning Agent, and Titer Plates) for a total of 15 x 96 antibody titer tests.
Product is not intended or validated for use in the diagnosis of disease or other conditions.
| Specifications | Description |
|---|---|
| Combined size (As installed - Instrumentation, HEPA filter, Workstation cart with monitor)* |
|
| Instrument chassis (Cydem VT liquid handler) |
|
| Workstation cart size** |
|
| Weight (As installed - Instrumentation, HEPA filter, Workstation cart with monitor) |
|
*Monitor and mounting arm positioned at a 45° angle.
**Workstation cart is always included in the Cydem VT system.
|
Front View 
|
Side View 
|
Front View Open 
|
Explore Cydem VT Models
Technical Documents
FAQ on the Cydem VT Automated Clone Screening System
What is the throughput of the Cydem VT system?
The Cydem VT Automated Clone Screening System can screen up to 96 clones in parallel shaken 5 mL microtiter plate format.
Which types of monoclonal antibodies (mAbs) can the Cydem VT system detect?
The Cydem VT system uses Valita Titer Assay technology and can quantify all Fc-containing molecules (e.g., IgG-based antibodies IgG 1, 2, 3, and 4 subclasses, standard, bispecific, fusion protein formats, etc.).
What parameters does the Cydem VT system measure during clone and minipool screening?
The system provides regular on-deck measurements of pH and dissolved oxygen (DO) values, monoclonal antibody (mAb) titer, and cell count and viability.
What cultivation modes does the Cydem VT system support?
The Cydem VT system allows for a pH-controlled fed-batch cultivation for 96 clones, with well-specific gassing control for pH (CO2) and dissolved oxygen (DO) control. The liquid handler can add liquid base for additional pH control.
What cell lines does the Cydem VT system support?
The Cydem VT system supports the shaken cultivation of Chinese Hamster Ovary (CHO) cells.
What is the working volume of the Cydem VT system bioreactor plates?
The working volume is in a range of 3-5 mL.
