CytoFLEX nano級流式細胞儀
CytoFLEX nano 流式細胞分析儀是 CytoFLEX 平台的最新成員。.
CytoFLEX nano 流式細胞儀專為分析奈米粒子而設計，它克服了傳統流式細胞儀的限制，為研究人員提供了靈敏, 一致且靈活的解決方案，有助於他們推進研究。
CytoFLEX nano 流式細胞儀為流式細胞儀學的重要發展，可讓使用者輕鬆分析至少小至 40 nm* 的細胞外小泡 (EV)，同時提供多達 6 個獨立的螢光檢測通道及 5 個側向散射通道，以提供完整的特性分析，並為您的奈米粒子研究開闢更多的途徑。
CytoFLEX nano Flow Cytometer Features
Explore the full picture of your EV experiment with the CytoFLEX nano Flow Cytometer. This purpose-built flow cytometer provides greater sensitivity, consistent instrument performance, and flexibility to study your sample.
靈敏度
靈活性
最大一致性
熟悉性
The CytoFLEX nano analyzer utilizes CytExpert for CytoFLEX Acquisition and Analysis Software and has the ability to count, size, and characterize EVs using a single technique, propelling your research forward.
| Size | Count | Characterize |
| Particle size determination by flow cytometry requires calibration using an appropriately characterized reference particle paired with a calibration method. Latex polystyrene particles are the tool of choice for this task due to their NIST-traceable nature and reliably consistent manufacturing. Through the calibration method taught to each CytoFLEX nano flow cytometer customer, data will be reliably reportable in absolute units for method comparison and correlation. | The most robust counting methods rely on highly controlled volumetric delivery of samples rather than hand loading samples with the implied variability. The CytoFLEX nano flow cytometer allows users to customize their sample handling so that the right amount of sample is used for the results needed. | The ability to describe nanoscale particles requires best-in-class sensitivity. To make this possible, the CytoFLEX nano flow cytometer has completely redesigned the optics and fluidics system to maximize the detection of even the smallest amounts of fluorescence across 6 detection channels and excited by 4 collinear lasers. |
性能特性
| CytoFLEX nano Flow Cytometer Performance | ||
| Violet side scatter sensitivity | VSSC1: 40 nm relative to polystyrene nanoparticles VSSC2: 80 nm relative to polystyrene nanoparticles |
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| Scatter detection dynamic range | VSSC 1 for small range 40-150 nm polystyrene nanoparticles VSSC2 for large range 80-1000 nm polystyrene nanoparticles |
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| Violet forward scatter sensitivity | 300 nm relative to polystyrene nanoparticles | |
| Fluorescence sensitivity and resolution |
Simultaneous detection of fluorescence on six fluorescence detectors, using 500 nm CytoFLEX nano Multi-intensity Fluorospheres V447: 8 peaks B531: 8 peaks Y595: 8 peaks R670: 6 peaks R710: 5 peaks R792: 4 peaks |
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| Fluorescence rCV | CV < 10% (using QC Fluorospheres at 1 µL/min) | |
| Carryover between Samples | ≤1%a | |
| Acquisition speed | Maximum electronic acquisition speed | 16000 events/second with ≤ 95% yield |
| Recommended maximum sample acquisition speed | 5000 events/second to avoid possible swarming or coincidence situations. | |
| Volumetric Counting accuracy | > 90%b | |
Learn more about nanoscale flow cytometry
Explore CytoFLEX nano Models
a. Tested with polystyrene nanoparticles
b. Tested with 144 nm QC Scatterspheres on 3 units under the conditions: record 3 minutes at the sample flow rates of 1µL/min, 2µL/min, 3µL/min, 4µL/min, 5µL/min, 6µL/min respectively, repeat 5 times, then calculate the average total events for each speed, then compare the calculated volumetric counting accuracy with the theoretical total events ( the known concentration 1.3*10 8 x volume).
*polystyrene when triggering on violet side scatter
** In development. Performance characteristics have not been validated.
For Research Use Only. Not for use in diagnostic procedures.
