近垂直離心管轉子

近垂直離心管轉子的功能特色為短路徑長度、快速運行時間以及優異的分辨率。

近垂直離心管稍微偏離垂直約 7.5 – 9 度。此一特性創造了得以進行分離的較短路徑長度。較短路徑長度意味著較短的分離時間。

輕微偏離的角度將沉澱物推至離心管底部，於是，停止運行時，溶液不會接觸沉澱的雜質。

MLN-80 近垂直離心管轉子

最大 RPM – 80 000
最大 RCF - 389 000 x g
k 因子 - 20
離心管數量/體積/大小/尺寸
- 8 x 8 mL
- 16 x 58 mm
轉子容量 - 64 mL

NVT 100 近垂直離心管轉子

最大 RPM – 100 000
最大 RCF -750 000 x g
k 因子 - 8
離心管數量/體積/大小/尺寸：
- 8 x 5.1 mL
- ½ x 2 in
- 13 x 51 mm
容量 – 40.8 mL

NVT 65 近垂直離心管轉子

最大 RPM – 65 000
最大 RCF – 402 000 x g
k 因子 - 21
離心管數量/體積/大小/尺寸
- 8 x 13.5 mL
- 5/8 x 3 in
- 16 x 76 mm
轉子容量 – 108 mL

NVT 65.2 近垂直離心管轉子

最大 RPM – 65 000
最大 RCF – 416 000 x g
k 因子 - 15
離心管數量/體積/大小/尺寸
- 16 x 5.1 mL
- ½ x 2 in
- 13 x 51 mm
轉子容量 – 81.6 mL

NVT 90 近垂直離心管轉子

最大 RPM – 90 000
最大 RCF - 645 000 x g
k 因子 - 10
離心管數量/體積/大小/尺寸
- 8 x 5.1 mL
- ½ x 2 in
- 13 x 51 mm
轉子容量 – 40.8 mL

TLN-100 近垂直離心管轉子

最大 RPM – 100 000
最大 RCF - 450 000 x g
k 因子 - 14
離心管數量/體積/大小/尺寸
- 8 x 3.9 mL
- 13 x 38 mm
轉子容量 – 31.2 mL

TLN-120 近垂直離心管轉子

最大 RPM – 120 000
最大 RCF - 585 000 x g
k 因子 - 7
離心管數量/體積/大小/尺寸
- 8 x 1.2 mL
- 8 x 35 mm
轉子容量 – 9.6 mL

FAQ on Near-Vertical Rotors

What is the difference between a near-vertical rotor and a fixed-angle rotor?

A fixed-angle rotor is typically optimized for rapid pelleting applications, while a near-vertical rotor is often chosen for density gradient and isopycnic centrifugation workflows. Near-vertical rotors provide a shorter path length within the gradient, which can help reduce separation times for equilibrium and gradient-based applications.

Why are near-vertical rotors used for density gradient centrifugation?

Near-vertical rotors provide a shorter sedimentation path than many other rotor designs. This can reduce run times and support efficient density gradient separations while maintaining resolution between particles with different buoyant densities.

Why choose a near-vertical rotor for DNA purification?

Near-vertical rotors are commonly used in density gradient-based nucleic acid separation workflows because they can support efficient migration of DNA molecules through the gradient while reducing run times compared with some traditional rotor configurations.

What is a near-vertical rotor?

A near-vertical rotor is an ultracentrifuge rotor that holds sample tubes at a shallow angle between vertical and fixed-angle orientations. This geometry creates a shorter sedimentation path, helping reduce centrifugation times while maintaining high separation efficiency.

What applications are near-vertical rotors commonly used for?

Near-vertical rotors are frequently used for density gradient centrifugation, isopycnic separations, DNA purification, viral particle isolation, lipoprotein analysis, and other high-resolution ultracentrifugation workflows.

What is isopycnic centrifugation?

Isopycnic centrifugation is a density gradient separation technique in which particles migrate until they reach a position where their density matches the density of the surrounding gradient medium. Near-vertical rotors are commonly used for these applications because they can support efficient equilibrium separations.