OptiMATE Gradient Maker 自動化密度梯度溶液製備儀

用於密度梯度離心樣品溶液製備

OptiMATE Gradient Maker 自動化密度梯度溶液製備儀可將幾乎所有離心前步驟 (包括梯度製備、精確分液和離心管封口) 自動化，從而將費力且容易出錯的 密度梯度超高速離心法 density gradient ultracentrifugation (DGUC) 過程簡化為易於維護的操作，大幅縮短運行時間，且不會犧牲純度或產量。

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Resources + Tools
Download Brochure Technical Documents Density Gradient Media OptiMATE Density Gradient Calculator OptiXTRACT Sample Recovery Aid
OptiMATE gradient maker for density gradient centrifugation
OptiMATE gradient maker for density gradient centrifugation

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OptiMATE 自動化密度梯度溶液製備儀的特點

自動化分裝與封管

預製漸層

  • 能夠製備線性和階梯梯度
  • 線性梯度的運行時間大幅縮短 (減少 約75% )

簡易圖形介面 (GUI)與快速方法(method)建立功能

  • 訓練新操作員只需數小時，而非數星期
  • 已發佈的方法可確保不同操作人員與不同批次間的一致性
  • 內建梯度分佈即時可視化功能

介紹OptiMATE 自動化密度梯度溶液製備儀

 


自動分裝與離心管封口

預製漸層

簡單圖形介面與方法建立

自動化、一致且有效率的梯度分配功能


  • 可自動分配階梯式與線性梯度，並自動封管

  • 從預先形成的線性梯度開始，可大幅縮短運行時間

  • 改善一致性 - 透過自動化減少錯誤

  • 可在短短數小時內產生高品質密度梯度溶液

  • 與 氯化銫 cesium chloride (CsCl), 碘克沙醇 iodixanol (IDX), 和蔗糖相容
OptiMATE Gradient Maker performance data

雖然梯度分佈圖顯示即使僅離心 4 小時也有足夠的分離效果，但為了提升條帶解析度與清晰度，控制組的預製梯度實驗（左圖）與 AAV 的預製梯度實驗（右圖）皆延長至 5 小時。

瞭解更多

Resources

使用 OptiMATE 自動化密度梯度溶液製備儀快速自動純化腺相關病毒(Adeno-Associated Virus)

 

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使用 OptiMATE 自動化密度梯度溶液製備儀降低病毒載體(Viral Vector)純化的變異性並減少人工操作時間

 

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OptiMATE Gradient Maker
功能概述

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Explore More Solutions for Density Gradient Centrifugation

1
Media preparation

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Gradient generation

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3
Centrifugation

Optima XPN Ultracentrifuge
4
Band extraction

OptiXTRACT Sample Recovery Aid

Technical Documents

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FAQ on the OptiMATE Gradient Maker

What is density gradient centrifugation?

Density gradient ultracentrifugation (DGUC) is a centrifuge-based technique for providing superior purification of viral vectors (e.g., isolating full AAV particles from partial and empty capsids), along with other materials (such as plasmid DNA) in gene therapy production workflows. DGUC results in a layered gradient based on the density of materials in the original sample.

What applications is density gradient ultracentrifugation used for?

  • Viruses / viral vectors (e.g., AAV)
  • Virus-like particles and bacteriophage
  • Extracellular vesicles (EVs)
  • Proteins / protein complexes
  • Lipid nanoparticles
  • Synthetic nanoparticles or other nanomaterials
  • Nucleic acids / plasmids
  • Lipemic separations

What density gradient reagents are compatible with the OptiMATE Gradient maker?

  • Cesium Chloride
  • Iodixanol (OptiPrep™)
  • Sucrose

What tubes are compatible with the OptiMATE Gradient Maker?

Sizes

  • 16 mm (8.9 to 13.5 mL)
  • 25 mm (29.9 to 39 mL)
  • 38 mm (94 mL)
Formats
  • Quick-Seal
  • OptiSeal
  • Open-Top
Materials
  • Polypropylene
  • Ultra-Clear
Filling and sealing performance are impacted by tube material, tube size, and tube volume. Not all tubes meeting the above criteria may be suitable. For a complete list of compatible rotors and tubes, please see the instrument brochure and instructions for use (IFU).

Are the tubing lines reusable?

Yes, tubing lines are reusable. They have been tested to three runs.

Can I use 3rd-party tubes with the OptiMATE Gradient Maker?

No, OptiMATE is only compatible with ultracentrifuge tubes provided by Beckman Coulter Life Sciences.