垂直角度轉子
垂直角度離心管轉子功能特色為任何種類轉子之中路徑長度最短、最快速運行時間以及最高的分辨率。
在垂直轉子運行時，離心管為垂直。這些轉子的短路徑長度與低 K 因子意味著顆粒與沉澱物的行程距離很短，從而減少了應用的運行時間。
這不是用於沉澱最好的轉子，因為在完全垂直離心管中的離心力讓沉澱物分佈在整個離心管的壁上。
垂直轉子用於
- 等密度分離 – 特別是氯化銫中的 DNA 條帶
- 梯度與密度緩衝墊分離，例如用於病毒顆粒分離
梯度密度緩衝墊分離：
- 增加停止於離心管底部高密度緩衝墊的密度層
- 最適合在“較軟”的緩衝墊面上分離靈敏的沉澱樣本
FAQ on Vertical Angle Rotors
What applications are vertical rotors commonly used for?
Vertical rotors are commonly used for isopycnic centrifugation, density gradient separations, plasmid DNA purification, viral particle isolation, lipoprotein analysis and other ultracentrifugation workflows that require rapid equilibrium separations.
What is a vertical rotor?
A vertical rotor is an ultracentrifuge rotor that maintains sample tubes parallel to the axis of rotation during centrifugation. This geometry creates the shortest sedimentation path of any rotor design and helps accelerate density gradient separations.
Why are vertical rotors used for isopycnic centrifugation?
Vertical rotors provide a very short path length through the density gradient. This allows particles to migrate quickly to their equilibrium density, helping reduce run times while maintaining high separation resolution.
What is the k-factor of a centrifuge rotor?
The k-factor is a measure of a rotor's sedimentation efficiency. Lower k-factor values generally indicate faster particle sedimentation and shorter run times. When comparing ultracentrifuge rotors, the k-factor can help determine which rotor may provide the most efficient separation for a specific application.
Are vertical rotors suitable for pelleting applications?
Vertical rotors are generally not the preferred choice for pelleting. Because the tubes remain vertical during centrifugation, pellets may spread along the outer wall of the tube rather than forming a compact pellet at the bottom. Fixed-angle or swinging-bucket rotors are typically better suited for pelleting workflows.
What is the difference between a vertical rotor and a fixed-angle rotor?
Vertical rotors are primarily designed for density gradient and isopycnic separations, where particles move through a gradient to reach their equilibrium density. Fixed-angle rotors are typically optimized for pelleting applications, where particles sediment toward the tube wall and form a pellet. The choice depends on whether the goal is particle pelleting or high-resolution gradient separation.
When should I use a near vertical rotor instead of a vertical rotor?
Use a near vertical rotor instead of a vertical rotor if particles in your solution will pellet out of solution and risk contaminating your gradient bands. In a near vertical rotor, those containments will move to the bottom of the tube instead of remain on the side wall, as they do in a vertical rotor.
