絕對計數微球試劑盒

淋巴細胞亞群的細胞絕對數和百分比值通常使用自動血球計數儀器上的白血球數和淋巴細胞數乘以流式細胞儀上測量的陽性染色細胞百分比。然而，這種標準（間接）方法卻存在各個實驗室間的變動差異。1-4 流式計數螢光微球是這種絕對計數標準（間接）方法的有效替代選擇，通過單平台流式免疫表型分析直接提供絕對計數結果。當生物標本和流式計數螢光微球的相同體積存在時，將確定試樣中細胞與螢光微球的比例，目標細胞和螢光微球隨後通過流式細胞測定。
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AQUIOS Flow-Count Fluorospheres

 AQUIOS Flow-Count Fluorospheres is a fluorescent microsphere reagent for direct determination of leukocyte absolute counts in biological specimens on the AQUIOS CL flow cytometer. Flow-Count Fluorospheres are a suspension of fluorescent microbeads used to determine absolute counts on the flow cytometer. Each fluorosphere contains a dye which has a fluorescent emission range of 525 nm to 700 nm when excited at 488 nm. They have uniform size and fluorescence intensity, and an assayed concentration allowing a direct determination of absolute counts.

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