從 FFPE 組織中萃取 DNA 和 RNA

FormaPure XL Total 核酸萃取試劑組

使用以 SPRI 磁珠化學技術從福馬林固定石蠟包埋組織樣本中萃取 DNA 和 RNA。

FormaPure XL 試劑組根據您的應用和實驗通量需求提供多種試劑組大小規格和萃取類型。為了獲得盡可能大限度的便利性,您還可從單個供應商處獲得對試劑和自動化方法的專業技術支援。

  • 可擴充的萃取方法,與手動和自動化處理相容(包括樣本脫蠟和裂解)
  • 簡單工作流程,手動操作時間短
  • 具有靈活性,可因應不同樣本數量
  • 產量突出、經驗證的完整性和萃取純度
  • 經各種樣品和組織類型試驗驗證的可靠效能
  • 相容 7x 10 μM 薄片

Explore From FFPE Tissue Models

FormaPure XL Total 核酸萃取試劑組工作流程圖  

Genomics FormaPure Total XL Workflow

產品規格明細

Application Uses Nucleic Acid Sample Prep, Total NA Extraction, Total NA Isolation, Total NA Sequencing
Format Liquid
Starting Sample Material FFPE (formalin fixed paraffin embedded) tissue.
Automated Available Yes
Item Specifications Referenced C35991

Content and Resources

AMP 2019: Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples In this poster, presented at AMP 2019, we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.
Simultaneously DNA and RNA extraction from formalin-fixed paraffin embedded (FFPE) tissue In this poster we present a streamlined NGS ready to use sample prep workflow for FFPE tissue using a product in current development, FormaPure XL Total* (Beckman Coulter Life Science). FormaPure XL Total uses SPRI (Solid Phase Reverse Immobilization) paramagnetic beads to isolate DNA and RNA simultaneously.
Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples In this poster we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.
A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System This application note demonstrates the use of FormaPure XL Total, a total nucleic acid extraction kit for FFPE samples, in conjunction with the KingFisher™ Duo Prime Sample Purification System as a potential solution that mitigates some of the challenges with FFPE workflows.
Manual or Automated RNA, DNA or Total Nucleic Acid Extraction from FFPE Tissues FormaPure XL reagent kits represent a single-chemistry system with demonstrated capability for use in Next-Generation Sequencing (NGS) as well as other downstream applications including qPCR. Maximizing integrity, yield and purity from an FFPE sample is required to minimize the risk of losing important genetic information

Technical Documents

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