Resources about Wastewater Research

There has been a lot of research conducted since the beginning of the COVID-19 pandemic. Explore with the links below to learn more about the wastewater research for SARS-COV-2 surveillance:

General resources

Multiple Techniques and General Information 

Ultracentrifugation

  • Sampling and viral concentration for SARS-CoV-2 detection in wastewater. Veronica1, Antelo, Iraola Lab1. Posted: May 7, 2020.
    1Microbial Genomics Laboratory, Institut Pasteur Montevideo, Uruguay

  • Quantification of SARS-CoV-2 and cross-assembly phage from wastewater to monitor coronavirus transmission within communities. Hyatt Green1*, Maxwell Wilder1, Mary Collins2, Ariana Fenty1, Karen Gentile3, Brittany L. Kmush4, Teng Zeng5, Frank A. Middleton3 and David A. Larsen4*. Posted: May 23, 2020.
    1Department of Environmental and Forest Biology, SUNY-ESF, Syracuse, NY 13210
    2Department of Environmental Studies, SUNY-ESF, Syracuse, NY 13210
    3Department of Neuroscience and Physiology, Upstate Medical University, Syracuse, NY 13210
    4Department of Public Health, Syracuse University, Syracuse, NY 13244
    5Department of Civil and Environmental Engineering, Syracuse University, Syracuse, NY 13244

  • Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. Tulio M.Fumiana, José Paulo G. Leitea, Alejandro A. Castellob, Aldo Gaggeroc, Maria Susana L. de Cailloud, Marize P.Miagostovicha. Posted: August 30, 2010.
    a Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil
    b Laboratorio de Inmunología y Virología, Universidad Nacional de Quilmes Bernal, Argentina
    c Programa de Virología, ICBM, Facultad de Medicina, Universidad de Chile, Chile
    d Instituto de Microbiología, Universidad de Tucumán, Argentina

  • Development of wastewater pooled surveillance of SARS-CoV-2 from congregate living settings. Lisa M. Colosi1, Katie E. Barry2, Shireen M. Kotay2, Michael D. Porter1, Melinda D. Poulter3, Cameron Ratliff4, William Simmons4, Limor I. Steinberg2, D. Derek Wilson5, Rena Morse6, Paul Zmick4 and Amy J. Mathers2,3*. Posted: October 11, 2020.
    1Department of Engineering Systems & Environment, University of Virginia, Charlottesville, VA 22904, USA
    2Department of Medicine, Division of Infectious Diseases, University of Virginia Health System, Charlottesville, VA 22908, USA
    3Clinical Microbiology Laboratory, Department of Pathology, University of Virginia Health System, Charlottesville, VA 22908, USA
    4Facilities Management, Energy and Utilities Division, University of Virginia, Charlottesville, VA 22904, USA
    5Facilities Management, University of Virginia Health System, Charlottesville, VA 22908, USA
    6Health Information & Technology, University of Virginia Health System, Charlottesville, Virginia, USA

  • Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater. Warish Ahmed*, Paul M. Bertsch, Aaron Bivins, Kyle Bibby, Kata Farkas, Amy Gathercole, Eiji Haramoto, Pradip Gyawali, Asja Korajkic, Brian R. McMinn, Jochen F. Mueller, Stuart L. Simpson, Wendy J.M. Smith, Erin M. Symonds, Kevin V. Thomas, Rory Verhagen, and Masaaki Kitajimal. Posted: October 15, 2020.
    *Corresponding author at: Ecosciences Precinct, 41 Boggo Road, Dutton Park 4102, Queensland, Australia. For additional authors affiliations, please refer to the author information in the article.  

  • The efficiency of concentration methods used to detect enteric viruses in anaerobically digested sludge. Tatiana Prado1*, Wilma de Carvalho Pereira Bonet Guilayn2, Ana Maria Coimbra Gaspar3, and Marize Pereira Miagostovich1. Posted: Feb 2013.
    1Laboratório de Virologia Comparada e Ambiental
    2Departamento de Saneamento e Saúde Ambiental, Escola Nacional de Saúde Pública-Fiocruz, Rio de Janeiro, RJ, Brasil
    3Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil

Ultrafiltration

  • Correlation of SARS-CoV-2 RNA in wastewater with COVID-19 disease burden in sewersheds (Utah study). Jennifer Weidhaasa, Zachary T. Aanderudb, D. Keith Roperc, James VanDersliced, Erica Brown Gaddise, Jeff Ostermillere, Ken Hoffmane, Rubayat Jamala, Phillip Heckf, Yue Zhangg, Kevin Torgersenb, Jacob Vander Laane, and Nathan LaCrossh. Posted: June 25, 2020.
    aUniversity of Utah, Civil and Environmental Engineering, 110 Central Campus Drive, Suite 2000, Salt Lake City, UT, USA
    bBrigham Young University, Department of Plant & Wildlife Sciences, Provo, UT, USA
    cUtah State University, Biological Engineering, 4105 Old Main Hill, Logan, UT, USA
    dUniversity of Utah, Family and Preventive Medicine, 275 Chipeta Way, Suite A, Salt Lake City, UT, USA
    eUtah Department of Environmental Quality, Division of Water Quality, 195 N 1950 West, Salt Lake City, UT, USA
    fCentral Valley Water Reclamation Facility, 800 West Central Valley Road, Salt Lake City, UT, USA
    gUniversity of Utah, School of Medicine, Department of Internal Medicine, Division of Epidemiology, 295 South Chipeta Way, Salt Lake City, UT, USA
    hUtah Department of Health, Division of Disease Control and Prevention, Bureau of Epidemiology, 288 N 1460 W, Salt Lake City, UT, USA

  • First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan. Eiji Haramotoa, Bikash Mallaa Ocean Thakalib, Masaaki Kitajimac. Posted: October 1, 2020.
    aInterdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
    bEnvironmental and Social System Science Course, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
    cDivision of Environmental Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan

  • The wastewater microbiome: A novel insight for COVID-19 surveillance. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñozab, Gustavo Núñez-Acuñaab, Diego Valenzuela-Mirandaab, Bárbara P.Benaventel, Constanza Sáez-Vera, Homero Urrutia, Beatriz Novoa, Antonio Figueras, Steven Roberts, Paulina Assmann, Marta Bravo. Posted: October 9, 2020.
    a Interdisciplinary Center for Aquaculture Research, University of Concepción, O'Higgins 1695, Concepción, Chile
    b Laboratory of Biotechnology and Aquatic Genomics, Center of Biotechnology, Barrio   Universitario s/n, University of Concepción, Concepción, Chile
    For additional authors affiliations, please refer to the author information in the article. 

  • Standard Operating Procedure for Sampling, Concentration and Detection of SARS-CoV-2 in Sewage published by Joan Rose, PhD, et al. Matthew Flood, Nishita D’Souza, Tiong Gim, and Joan Rose. Posted: October 17, 2020.

  • First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA. Samendra P.Sherchana, Shalina Shahina, Lauren M. Warda, Sarmila Tandukarb, Tiong G. Awa, Bradley Schmitzc, Warish Ahmedd, Masaaki Kitajimae. Posted: November 15, 2020.
    aDepartment of Environmental Health Sciences, Tulane University, 1440 Canal Street, Suite 2100, New Orleans, LA 70112, USA
    bInterdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan
    cLoudoun Water, 44865 Loudoun Water Way, Ashburn, VA 20147, USA
    dCSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia
    eDivision of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan

Chemical/Biochemical Precipitation

  • Metropolitan wastewater analysis for COVID-19 epidemiological surveillance. Walter Randazzoab, Enric Cuevas-Ferrandoa, Rafael Sanjuánc, Pilar Domingo-Calapcd, Gloria Sáncheza. Posted: September 1, 2020
    a Department of Preservation and Food Safety Technologies, Instituto de Agroquímica y Tecnología de Alimentos – Consejo Superior de Investigaciones Científicas (IATA-CSIC), Av. Agustín Escardino 7, Paterna, 46980, Valencia, Spain
    b Department of Microbiology and Ecology, Universitat de València, Av. Dr. Moliner, 50, Burjassot, 46100, Valencia, Spain
    c Institute for Integrative Systems Biology, I2SysBio, Universitat de València-CSIC, 46980, Paterna, Spain
    d Department of Genetics, Universitat de València, 46980, Paterna, Spain

     

*Corresponding author