21 CFR Part 11 Data Integrity for On-line WFI Instruments
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- 17-Marker, 18-Color Human Blood Phenotyping Made Easy with Flow Cytometry
- 21 CFR Part 11 Data Integrity for On-line WFI Instruments
- 8011+ Reporting Standards Feature and Synopsis
- Air Particle Monitoring ISO 21501-4 Impact
- Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi‑CELL XR
- Biomek Automated NGS Solutions Accelerate Genomic Research
- Biomek i-Series Automation of the Beckman Coulter GenFind V3 Blood and Serum DNA Isolation Kit
- Preparation and purification of carbon nanotubes using an ultracentrifuge and automatic dispensing apparatus, and analysis using an analytical centrifuge system
- Viability Assessment of Cell Cultures Using the CytoFLEX
- Classifying a Small Cleanroom using the MET ONE HHPC 6+
- Clean Cabinet Air Particle Evaluation
- Recommended cleaning procedure for the exterior surface of the MET ONE 3400+
- Counting Efficiency: MET ONE Air Particle Counters and Compliance to ISO-21501
- Efficient kit-free nucleic acid isolation uses a combination of precipitation and centrifugation separation methods
- Echo System-Enhanced SMART-Seq v2 for RNA Sequencing
- Grading of nanocellulose using a centrifuge
- Grading of pigment ink and measurement of particle diameter using ultracentrifugation / dynamic light scattering
- How to Use Violet Laser Side Scatter Detect Nanoparticle
- HRLD Recommended Volume Setting
- Particle Size Analysis Simple, Effective and Precise
- MET ONE Sensor Verification
- Metal colloid purification and concentration using ultracentrifugation
- Separation and purification of metal nanorods using density gradient centrifugation
- Miniaturized and High-Throughput Metabolic Stability Assay Enabled by the Echo Liquid Handler
- Modern Trends in Non‐Viable Particle Monitoring during Aseptic Processing
- Particle diameter measurement of a nanoparticle composite - Using density gradient ultracentrifugation and dynamic light scattering
- Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice
- Particle testing in cleanroom high-pressure gas lines to ISO 14644 made easy with the MET ONE 3400 gas calibrations
- Pharma Manufacturing Environmental Monitoring
- Pharma Manufacturing Paperless Monitoring
- Analysis of plant genome sizes using flow cytometry: a case study demonstrating dynamic range and measurement linearity
- Calibrating the QbD1200 TOC Analyzer
- Detection Limit
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- A fully automated plate-based optimization of fed-batch culture conditions for monoclonal antibody-producing CHO cell line
- A Deeper Look at Lipid Nanoparticles
- A High-Throughput, Automated Screening Platform for IgG Quantification During Drug Discovery and Development
- Automated Research Flow Cytometry Workflow Using DURA Innovations Dry Reagent Technology with the *Biomek i7 Automated Workstation and *CytoFLEX LX Flow Cytometer
- Automating antibody titration using a CytoFLEX LX analyzer Integrated with a Biomek i7 Multichannel workstation and Cytobank streamlined data analysis
- Monitoring Yeast Cultures with the BioLector and Multisizer 4e instruments
- Determination of cell death in the BioLector Microbioreactor
- Echo System-Enhanced SMART-Seq v4 for RNA Sequencing
- Leveraging Analytical Ultracentrifugation for Comprehensive Characterization of Lipid Nanoparticles in Drug Delivery Systems| 1Accelerating Answers Background Analytical ultracentrifugation (AUC) is a well-established biophysical technique for the characterization of macromolecules. It has become the gold standard characterization method for adeno-associated viruses (AAVs) to determine particle loading states1 and has recently been applied to characterize lipid nanoparticles (LNPs) using sedimentation velocity and density contrast methods.2,3 Both methods provide information on the purity and comparability of different formulations. Density contrast AUC is used to experimentally determine the sample's density or partial specific volume distribution, enabling the characterization of unloaded particles, RNA copy number in the LNPs, and size distributions. AUC provides an array of unique information from a single technique, opens new possibilities, and enhances the characterization of LNPs for safer therapeutics.APPLICATION NOTE
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